Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury
Acta cir. bras
;
29(3): 186-192, 03/2014. graf
Artigo
em Inglês
| LILACS
| ID: lil-703525
ABSTRACT
To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion. METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm 2.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Estresse Oxidativo
/
Isquemia
/
Pulmão
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Centro Universitário São Camilo/BR
/
Universidade Federal da Grande Dourados/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de Mato Grosso do Sul/BR
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