Use of P450 cytochrome inhibitors in studies of enokipodin biosynthesis
Braz. j. microbiol
;
44(4): 1285-1290, Oct.-Dec. 2013. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-705269
ABSTRACT
Enokipodins A, B, C, and D are antimicrobial sesquiterpenes isolated from the mycelial culture medium of Flammulina velutipes, an edible mushroom. The presence of a quaternary carbon stereocenter on the cyclopentane ring makes enokipodins A-D attractive synthetic targets. In this study, nine different cytochrome P450 inhibitors were used to trap the biosynthetic intermediates of highly oxygenated cuparene-type sesquiterpenes of F. velutipes. Of these, 1-aminobenzotriazole produced three less-highly oxygenated biosynthetic intermediates of enokipodins A-D; these were identified as (S)-(-)-cuparene-1,4-quinone and epimers at C-3 of 6-hydroxy-6-methyl-3-(1,2,2-trimethylcyclopentyl)-2-cyclohexen-1-one. One of the epimers was found to be a new compound.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Sesquiterpenos
/
Flammulina
/
Anti-Infecciosos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Japão
Instituição/País de afiliação:
Hokkaido University/JP
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