Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
Mem. Inst. Oswaldo Cruz
;
109(2): 236-245, abr. 2014. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-705811
ABSTRACT
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tuberculose Bovina
/
Técnicas de Tipagem Bacteriana
/
Técnicas de Genotipagem
/
Mycobacterium bovis
Tipo de estudo:
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
País/Região como assunto:
América do Sul
/
Argentina
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2014
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Argentina
Instituição/País de afiliação:
National University of Mar del Plata/AR
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