Evaluation of polymerase chain reaction (PCR) for identifying Mycobacterium bovis isolates from the modified Middlebrook 7H11 agar thin layer technique
Rev. Inst. Adolfo Lutz
;
71(4): 753-756, out.-dez. 2012. tab
Artigo
em Inglês
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-706148
ABSTRACT
The associated use of the modified Middlebrook 7H11 agar thin layer technique and the Polymerase Chain Reaction (PCR) assay enabled to perform the early identification of microcolonies of Mycobacterium bovis from 12th to 25th day of culture. In order to reduce the time for performing the Mycobacterium bovis identification, the combined use of these two techniques was evaluated by analyzing the microcolonies of mycobacteria at the 8th day after culturing. Until the last day of analysis, all of the PCR-positive samples already showed the microcolonies. Therefore, the early diagnosis of bovine tuberculosis is feasible, without an apparent macroscopic colonies growth.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tuberculose
/
Reação em Cadeia da Polimerase
/
Técnicas e Procedimentos Diagnósticos
/
Ágar
/
Mycobacterium bovis
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Rev. Inst. Adolfo Lutz
Ano de publicação:
2012
Tipo de documento:
Artigo
Instituição/País de afiliação:
Agência Paulista de Tecnologia dos Agronegócios/BR
/
Universidade de São Paulo/BR
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