Confocal laser scanning microscopy and immunohistochemistry of cerebellar Lugaro cells
Biocell
;
37(2): 29-36, ago. 2013. ilus
Artigo
em Inglês
| LILACS
| ID: lil-708030
ABSTRACT
The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fibers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors that regulate calcium levels. CaMKII alpha immunoreactivity of L ugaro cell soma and processes suggest its participation as a molecular switch for long-term information storage, and serving as a molecular basis of long-term synaptic memory. N-cadherin immunoreactivity was correlated with somato-somatic and somato-dendritic junctions between Lugaro cells and their synaptic connections.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Imuno-Histoquímica
/
Cerebelo
/
Microscopia Confocal
/
Interneurônios
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Venezuela
Instituição/País de afiliação:
Zulia University/VE
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