Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Braz. j. microbiol
;
45(1): 225-230, 2014. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-709456
ABSTRACT
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tuberculose Bovina
/
Reação em Cadeia da Polimerase
/
Técnicas Bacteriológicas
/
Técnicas de Diagnóstico Molecular
/
Carne
/
Mycobacterium bovis
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR
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