Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center
Rev. bras. hematol. hemoter
;
36(3): 202-207, May-Jun/2014. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-713677
ABSTRACT
OBJECTIVE:
To present the initial results of first three years of implementation of a genetic evaluation test for bone marrow-derived mesenchymal stem cells in a Cell Technology Center.METHODS:
A retrospective study was carried out of 21 candidates for cell therapy. After the isolation of bone marrow mononuclear cells by density gradient, mesenchymal stem cells were cultivated and expanded at least until the second passage. Cytogenetic analyses were performed before and after cell expansion (62 samples) using G-banded karyotyping.RESULTS:
All the samples analyzed, before and after cell expansion, had normal karyotypes, showing no clonal chromosomal changes. Signs of chromosomal instability were observed in 11 out of 21 patients (52%). From a total of 910 analyzed metaphases, five chromatid gaps, six chromatid breaks and 14 tetraploid cells were detected giving as total of 25 metaphases with chromosome damage (2.75%).CONCLUSION:
The absence of clonal chromosomal aberrations in our results for G-banded karyotyping shows the maintenance of chromosomal stability of bone marrow-derived mesenchymal stem cells until the second passage; however, signs of chromosomal instability such as chromatid gaps, chromosome breaks and tetraploidy indicate that the long-term cultivation of these cells can provide an intermediate step for tumorigenesis. .
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Citogenética
/
Instabilidade Cromossômica
/
Células-Tronco Mesenquimais
/
Cariotipagem
Tipo de estudo:
Estudo observacional
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Rev. bras. hematol. hemoter
Assunto da revista:
Hematologia
Ano de publicação:
2014
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Pontifícia Universidade Católica do Paraná/BR
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