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Synthesis and evaluation of artificial antigens for astragaloside IV
Yu, Sheng-lan; Ou-yang, Zhen; Xu, Jia-bing; Meng, Ting.
Afiliação
  • Yu, Sheng-lan; Jiangsu University. School of Food and Biological Engineering. Zhenjiang. CN
  • Ou-yang, Zhen; Jiangsu University. School of Food and Biological Engineering. Zhenjiang. CN
  • Xu, Jia-bing; Jiangsu University. School of Food and Biological Engineering. Zhenjiang. CN
  • Meng, Ting; Jiangsu University. School of Food and Biological Engineering. Zhenjiang. CN
Rev. bras. farmacogn ; 24(3): 282-287, May-Jun/2014. graf
Article em En | LILACS | ID: lil-719454
Biblioteca responsável: BR1.1
ABSTRACT
The objective of this study was to produce artificial antigens for astragaloside IV that could be used to prepare antibodies against astragaloside IV screened in Radix astragali (Astragalus membranaceus (Fisch) Bunge, Fabaceae) and its preparations, using an indirect ELISA. Astragaloside IV was coupled to carrier proteins, bovine serum albumin and ovalbumin using the sodium periodate method and was then evaluated using SDS-PAGE, MALDI-TOF MS and animal immunizations. The coupling ratio of astragaloside IV to bovine serum albumin ratio was determined to be thirteen, and the indirect ELISA demonstrated that three groups of mice immunized with astragaloside IV-bovine serum albumin produced anti-astragaloside IV- bovine serum albumin-specific antibody, with a minimum serum titer of 19600. A method for synthesizing highly immunogenic astragaloside IV artificial antigens was successfully developed thus indicating its feasibility in the establishment of a fast immunoassay for astragaloside IV content determination in Radix astragali and its products.
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Texto completo: 1 Índice: LILACS Idioma: En Revista: Rev. bras. farmacogn Assunto da revista: FARMACIA Ano de publicação: 2014 Tipo de documento: Article / Project document

Texto completo: 1 Índice: LILACS Idioma: En Revista: Rev. bras. farmacogn Assunto da revista: FARMACIA Ano de publicação: 2014 Tipo de documento: Article / Project document