Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation
Acta cir. bras
;
29(10): 658-666, 10/2014. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-725287
ABSTRACT
PURPOSE:
To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation.METHODS:
Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity.RESULTS:
A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate.CONCLUSION:
Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation. .
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Células-Tronco
/
Transplante de Células-Tronco
/
Polpa Dentária
/
Glucose
/
Glutamina
Tipo de estudo:
Estudos de avaliação
Limite:
Adolescente
/
Adulto
/
Humanos
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2014
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Sao Paulo/BR
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