Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry
Mem. Inst. Oswaldo Cruz
;
109(8): 1078-1080, 12/2014. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-732594
ABSTRACT
Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability. .
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Giardia lamblia
/
Viabilidade Microbiana
/
Metronidazol
/
Antiprotozoários
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2014
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Portugal
Instituição/País de afiliação:
Universidade do Porto/PT
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