Immunological assays employed for the elucidation of an histoplasmosis outbreak in São Paulo, SP
Braz. j. microbiol
;
45(4): 1357-1361, Oct.-Dec. 2014. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-741287
ABSTRACT
Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions in 50% of samples evaluated. The analysis of the second sample batch, collected two months after the exposure showed that 96.7% were reactive by DI with antibodies titers ranging from 1 to 16 and 100% of reactivity against H and M fractions, by IB, suggesting an acute infection. The analysis of the overall agreement between the methods showed to be reasonable (κ = 0.37). This study confirms the importance and efficacy of more sensitive methodologies, such as IB assay, to early elucidation of disease, especially in cases of patients without mycological information.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Surtos de Doenças
/
Técnicas Microbiológicas
/
Testes Diagnósticos de Rotina
/
Histoplasma
/
Histoplasmose
/
Anticorpos Antifúngicos
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Instituto Adolfo Lutz/BR
Similares
MEDLINE
...
LILACS
LIS