Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis
Braz. j. microbiol
;
46(2): 557-564, Apr-Jun/2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-749735
ABSTRACT
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Paratuberculose
/
Variação Genética
/
Mycobacterium avium subsp. paratuberculosis
/
Repetições Minissatélites
/
Tipagem Molecular
Limite:
Animais
País/Região como assunto:
América do Sul
/
Argentina
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2015
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Argentina
Instituição/País de afiliação:
Instituto Nacional de Tecnología Agropecuaria/AR
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