The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
Braz. j. med. biol. res
;
48(7): 637-643, 07/2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-751350
ABSTRACT
We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated ALP enzyme activity, and enhanced mineralized nodule formation. Stimulation with TNF-α led to enhanced interleukin-6 and nuclear factor-κB expression and downregulated markers of osteoblastic differentiation. 6-Gingerol reduced the degree of inflammation in TNF-α-treated MG-63 cells. In conclusion, 6-gingerol stimulated osteoblast differentiation in normal physiological and inflammatory settings, and therefore, 6-gingerol represents a promising agent for treating osteoporosis or bone inflammation.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Trissomia
/
Síndrome de Down
/
Recém-Nascido de muito Baixo Peso
/
Transtornos Cromossômicos
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Limite:
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Harbin Medicine University/CN
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