Expression analysis of notsh signaling pathway molecules in shed cultured in keratinocyte growth medium
Braz. j. oral sci
;
14(2): 135-140, Apr.-June 2015. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-755036
ABSTRACT
Aim:
To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).Methods:
RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green.Results:
Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7.Conclusions:
Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Células-Tronco
/
Dente Decíduo
/
Expressão Gênica
/
Queratinócitos
/
Meios de Cultura
/
Receptores Notch
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2015
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Malásia
Instituição/País de afiliação:
International Islamic Universiti Malaysia/MY
/
Universiti Sains Malaysia/MY
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