Expression analysis of notsh signaling pathway molecules in shed cultured in keratinocyte growth medium
Braz. j. oral sci
; 14(2): 135-140, Apr.-June 2015. tab, ilus
Article
em En
| LILACS
| ID: lil-755036
Biblioteca responsável:
BR218.1
ABSTRACT
Aim:
To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).Methods:
RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green.Results:
Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7.Conclusions:
Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Células-Tronco
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Dente Decíduo
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Expressão Gênica
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Queratinócitos
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Meios de Cultura
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Receptores Notch
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
Braz. j. oral sci
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
/
Project document