Newcastle diseasevaccineshitherto in vogue are produced from embryonated chickeneggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced eggproduction in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccinevirulence for safe vaccination by adapting the virus in a chickenembryofibroblastcell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean deathtime, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virusvaccine from the 18th(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doenças das Aves Domésticas
/
Vírus da Doença de Newcastle
/
Vacinas Atenuadas
/
Vacinas Virais
/
Galinhas
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Central Institute of Fisheries Technology/IN
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doenças das Aves Domésticas
/
Vírus da Doença de Newcastle
/
Vacinas Atenuadas
/
Vacinas Virais
/
Galinhas
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Central Institute of Fisheries Technology/IN
Consulta Detalhada
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