Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189
Braz. j. microbiol
;
46(4): 1147-1154, Oct.-Dec. 2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-769668
ABSTRACT
Abstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Pseudomonas aeruginosa
/
Piocinas
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
Paquistão
Instituição/País de afiliação:
Federal Urdu University of Arts, Science and Technology/PK
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