An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA
Mem. Inst. Oswaldo Cruz
;
111(2): 134-140, Feb. 2016. tab, graf
Artigo
em Inglês
| LILACS, SES-SP, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-772614
ABSTRACT
This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
DNA Viral
/
Vírus da Hepatite B
/
Hepatite B Crônica
/
Técnicas de Diagnóstico Molecular
/
Técnicas de Genotipagem
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Adolescente
/
Adulto
/
Idoso
/
Aged80
/
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Lactente
/
Masculino
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidade de São Paulo/BR
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