Purification and characterization of aminopeptidase activity from rat urine
Braz. j. med. biol. res
;
20(6): 759-61, 1987. tab
Artigo
em Inglês
| LILACS
| ID: lil-77431
ABSTRACT
The aminopeptidase activity of rats urine was tested using L-aminoacy1-2-naphtylamides and L-Leu-p-nitroanilide. The enzyme preparation was purified by ion-exchange and gel filtration chromatography and showed only a single active protein active protein band on 7.5% polyacrylamide gel electrophoresis. The enzyme was characterized by studying the effects of ions (divalent cations and chloride), chelating agents and -SH and -S-S- group reagents and by determining kinetic parameters (Km and Vmax for different substrates and K1 for amino acids, antibiotics and anti-inflammatory drugs)
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Índice:
LILACS (Américas)
Assunto principal:
Aminopeptidases
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1987
Tipo de documento:
Artigo
/
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