Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model
Acta cir. bras
;
31(3): 176-182, Mar. 2016. graf
Artigo
em Inglês
| LILACS
| ID: lil-777094
ABSTRACT
ABSTRACT PURPOSE:
To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model.METHODS:
Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8) Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis.RESULTS:
Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p<0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p<0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p<0.05).CONCLUSION:
Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Traumatismo por Reperfusão
/
Alopurinol
/
Precondicionamento Isquêmico
/
Substâncias Protetoras
/
Proteína HMGB1
/
Rim
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Wuhan University/CN
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