Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom
J. venom. anim. toxins incl. trop. dis
;
23: 2, 2017. tab, Ilus
Artigo
em Inglês
| LILACS
| ID: lil-794721
ABSTRACT
Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p< 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Venenos de Escorpião
/
Coração
Tipo de estudo:
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
/
Peru
Instituição/País de afiliação:
Federal University of Minas Gerais/BR
/
Universidad Nacional Mayor de San Marcos y Peru/PE
Similares
MEDLINE
...
LILACS
LIS