Restriction endonucleases from microorganisms isolated in Brazil: an isoschizomer of Hae III from a thermophilic Bacillus SP
Braz. j. med. biol. res
; 22(11): 1321-8, 1989. ilus
Article
em En
| LILACS
| ID: lil-82989
Biblioteca responsável:
BR26.1
RESUMO
The isolation and characterization of a restriction endonuclease from a thermophilic strain of Bacillus is described. The enzyme recognizes the palindromic sequence 5'...GGCC...3' as determined by PEI-cellulose chromatography of pancreativc DNAse and snake venom phosphodieterase digestion products of labelled DNA fragments, analysis of restriction digests and direct sequence analysis. The enzyme, denominated BspBR, is an isoschizomer of HaeIII and BspRI
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Índice:
LILACS
Assunto principal:
Bacillus
/
Enzimas de Restrição do DNA
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1989
Tipo de documento:
Article
/
Project document