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Aislamiento y caracterización parcial de la membrana plasmática de las células asciticas del carcinosarcoma de Walker-256 / Isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells
Acta cient. venez ; 40(4): 246-50, 1989. ilus, tab
Article em Es | LILACS | ID: lil-86870
Biblioteca responsável: VE1.1
ABSTRACT
Cell membranes of carcinosarcoma-256 ascites cells were isolated by the Zn++ method. Neutral sugars, sialic acid, fucose and N-Acetylhexosamines were determined in the cell membrane fraction. Cells were agglutinated by wheat germ agglutinin, Concanavalin A, and the lectins from Phaseolus vulgaris and Glycine max. No agglutination was observed with peanut lectin (Arachis hypogaea). Gel electrophoresis under dissociting conditions revealed more than 40 bands in the membrane fraction after silver staining. Only 2 bands were evident with PAS stainig
Assuntos
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Índice: LILACS Assunto principal: Líquido Ascítico / Células Tumorais Cultivadas / Carcinoma 256 de Walker / Fracionamento Celular Limite: Animals Idioma: Es Revista: Acta cient. venez Assunto da revista: CIENCIA Ano de publicação: 1989 Tipo de documento: Article
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Índice: LILACS Assunto principal: Líquido Ascítico / Células Tumorais Cultivadas / Carcinoma 256 de Walker / Fracionamento Celular Limite: Animals Idioma: Es Revista: Acta cient. venez Assunto da revista: CIENCIA Ano de publicação: 1989 Tipo de documento: Article