Comparaçäo entre ELISA com anticorpos policlonais e monoclonais para avaliaçäo do conteúdo de glicoproteina em vacina contra a raiva tipo Fuenzalida & OPalacios / Comparison betwen ELISA with polyclonal and monoclonal antibodies for evaluating glycoprotein content in \"Fuenzalida & Palacios\" rabies vaccine

ABSTRACT

Enzyme immunoassay is a valuable, rapid, and reproducible technique for the quantitative determination of rabies antigens in Fuenzalida & Palacios Rabies Vaccines. This study was performed in paltes sensitized with purified whole virus antibodies and the antigen seizured has been demonstrated with three different conjugates: anti whole total virus, anti-glycoprotein monoclonal and polyclonal antibodies conjugated woth peroxidase. The objective of this study was quantify the glycoprotein content of Fuenzalida & Palcios Rabies Vaccine and to compare thoses results wiwth the National Institute of Health Test (NIH)