HPLC determination of norepinephrine, 5-hydroxytyramine and 5-hydroxytryptamine in rat brain using sodium dodecyl sulphate as ion-pair

RESUMO

1. A simple HPLC method using sodium dodecyl sulfate (SDS) and elelctrochemical detection for the determination of norepinephrine (NE), 5-hydroxytyramine (DA) and 5-hydroxytryptamine (5-HT) in brain regions is described. 2. the tissue extraction procedure involved only two stepes: protein precipitation with perchloriac acid and pH adjustment to near 3.0. When used in combination with methanol as ion-pair in the mobile phase, sodium dodecyl sulgate effectively separated NE (6.6 min), epinephrine (EPI), 7.9 min), DA (14.3 min) and 5-HT (29.5 min) and the high methanol concentration used prevente the overlapping of the metabolites and monoamines in their chromatographic peaks. 3. the method was sensitive (40 to 70 pg of monoamine can be quantified) and reproducible and the values obtained for cortex, hippocampus, striatum and substantia nigra were consoant with most literature data