Identification T-cell reactive antigens in Nippostrongylus brasiliensis using a cell immunoblotting technique
Braz. j. med. biol. res
;
23(6/7): 573-6, 1990. ilus
Artigo
em Inglês
| LILACS
| ID: lil-92205
ABSTRACT
Antigens of the nematode Nippostrongylus brasiliensis were fractionated by SDS-PAGE under reducing conditions, transferred electrophoretically onto nitrocelluose and converted to antigen-bound nitrocellulose particles for use in vitro proliferation assays. Mesenteric lymph node cells from unfected rats were analyzed for reactivity against the fractionated antigens, revealing a range of different molecular weight antigens. Ian addition, when supernatants from these cultures were assyed for IL3, further reactive antigens wee detected. The results demonstrated that these approaches are useful for the identification of T-cell reactive components of a complex mixture of parasite antigens in helminth infections, where the cellular nature of protection is not well defined
Buscar no Google
Índice:
LILACS (Américas)
Assunto principal:
Técnicas In Vitro
/
Linfócitos T
/
Antígenos de Helmintos
/
Infecções por Nematoides
/
Nippostrongylus
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1990
Tipo de documento:
Artigo
/
Congresso e conferência
Similares
MEDLINE
...
LILACS
LIS