Indomethacin affects the development of tolerance to ethanol-induced diuresis

ABSTRACT

The present study analyzes the effects of indomethacin on the development of tolerance to the diuretic effect of ethanol. Male Wistar rats were with ethanol (2gKg-1 day-1) over a period of 4 weeks and the effect of ethanol on diuresis was assessed weekly. A significant tolerance to the diuretic effect of ethanol was detected after 3 weeks of treatment. However, rats treatment simultaneously with ethanol plus indomethacin (5mg/Kg, ip,, on alternate days) showed no tolerance to ethanol even after 4 weeks of treatment. The fact that indonethacin prevented the development of tolerance to the diuretic effect of ethanol but no influence on diuresis per se suggests that prostaglandins play a significant role in the mechanisms of tolerance to ethanol