Ingeniería genética en hongos filamentosos: clonamiento del gen de invertasa de neurospora crassa / Genetic engineering in filamentous fungi: cloning of the invertase gene from Neurospora crassa
Arch. biol. med. exp
;
23(2): 165-72, oct. 1990. tab
Artigo
em Espanhol
| LILACS
| ID: lil-96788
RESUMO
The invertase wild type gene of N. crassa was cloned into the YRp7 yeast vector. This recombinant plasmid was selected by functional complementation of an invertaseless mutant strain of S. cerevisiae. The isolated recombinant plasmid (named pNC2) carries a 7.6 Kb BamHI DNA fragment from N. crassa. The cloned DNA hydbridized with the N. crassa genomic DNA and transformed an invertase mutant of N. crassa Inv- to Inv+. Transformation of N. crassa Inv- to Inv+ seems to take at least two different integration events. One of them involves an integration closely linked to inv locus, and the other one apparently involves as integration of cloned DNA at a genomic site different that the inv locus
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Índice:
LILACS (Américas)
Assunto principal:
Clonagem Molecular
/
Genes Fúngicos
/
Glicosídeo Hidrolases
/
Neurospora crassa
Idioma:
Espanhol
Revista:
Arch. biol. med. exp
Assunto da revista:
Biologia
Ano de publicação:
1990
Tipo de documento:
Artigo
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