The dot-ELISA test for the detection of anti-PGL-1 IgM in leprosy patients and their contacts
Braz. j. med. biol. res
;
24(5): 441-8, 1991. tab
Artigo
em Inglês
| LILACS
| ID: lil-99475
RESUMO
Since dot-ELISA has recently been reported to be a sensitive, simple and method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for a antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. The sensitive of the techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93,4%). Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. These data indicate that dot-ELISA has a high spedificity and can be a useful tool in field evaluation
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Índice:
LILACS (Américas)
Assunto principal:
Imunoglobulina M
/
Ensaio de Imunoadsorção Enzimática
/
Glicolipídeos
/
Immunoblotting
/
Hanseníase
/
Antígenos de Bactérias
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1991
Tipo de documento:
Artigo
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