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The dot-ELISA test for the detection of anti-PGL-1 IgM in leprosy patients and their contacts
Braz. j. med. biol. res ; 24(5): 441-8, 1991. tab
Artigo em Inglês | LILACS | ID: lil-99475
RESUMO
Since dot-ELISA has recently been reported to be a sensitive, simple and method, we have compared it with the conventional microplate ELISA method. Sera of 124 leprosy patients, 136 household and professional contacts, and 92 controls were tested for a antibodies against a Mycobacterium leprae antigen using dot-ELISA on nitrocellulose membrane filters and microplate ELISA. The sensitive of the techniques was similar for multibacillary patients, but dot-ELISA was less sensitive for paucibacillary patients although it was more specific (100%) than ELISA (93,4%). Of 21 household contacts that gave a response by ELISA, 3 were also positive by dot-ELISA; one of these 3 developed indeterminate leprosy 12 months later and the other was diagnosed as borderline lepromatous after 28 months. These data indicate that dot-ELISA has a high spedificity and can be a useful tool in field evaluation
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Índice: LILACS (Américas) Assunto principal: Imunoglobulina M / Ensaio de Imunoadsorção Enzimática / Glicolipídeos / Immunoblotting / Hanseníase / Antígenos de Bactérias Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Humanos Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1991 Tipo de documento: Artigo

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Índice: LILACS (Américas) Assunto principal: Imunoglobulina M / Ensaio de Imunoadsorção Enzimática / Glicolipídeos / Immunoblotting / Hanseníase / Antígenos de Bactérias Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Limite: Humanos Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 1991 Tipo de documento: Artigo