The nifHDK operon in the free-living nitrogen-fixin bacteria azospirillum brasiliense sequentially comprises genes H, D, K, an 353 BP orf and gene Y
Braz. j. med. biol. res
;
24(7): 649-75, 1991. tab
Artigo
em Inglês
| LILACS
| ID: lil-99502
RESUMO
The complete nucleotide sequence of the nitrogenase structural genes form Azospirillum brasilense was determined. Two additional open reading frames of 353 and 683 base pairs were detected downstream of the nifK gene. Structures resembling the consensus nif promoter and NifA-binding motif were found only upstream from the nifH region and an inverted repeat structure locate downstream of the nifY gene may be a potential stem-and-loop transcriptional terminator. The nif structural genes of Azospirillum brasiliense are transcribed as a transcription unit and organized as nifHDK orf1 Y, NifH, NifD and NifK polypeptides share significant sequence identies when compared to nif structural gene products from other organisms. The three polypeptides are characterized by the presence of highly conserved cysteine residues which may play a role in binding the iron-sulfur cluster
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Índice:
LILACS (Américas)
Assunto principal:
Óperon
/
Proteínas de Bactérias
/
Azospirillum brasilense
/
Fixação de Nitrogênio
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1991
Tipo de documento:
Artigo
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