A reverse phase high performance liquid chromatography method for simultaneous estimation of melatonin, carbamazepine epoxide and carbamazepine simultaneously in serum.
Indian J Physiol Pharmacol
;
2006 Oct-Dec; 50(4): 427-30
Artigo
em Inglês
| IMSEAR
| ID: sea-107954
ABSTRACT
Monitoring of plasma antiepileptic drugs is useful for better clinical management in epileptic patients, particularly in children. Carbamazepine (CBZ) is one of the commonly prescribed anticonvulsants. The active metabolite of carbamazepine-carbamazepine-10-11 epoxide (CBZ-Epo) also exhibits anticonvulsant effect. The pineal hormone, melatonin exerts an anticonvulsant effect in experimental seizure models and recently has also been used in cases of childhood epilepsy. To facilitate the simultaneous plasma estimation of carbamazepine, carbamazepine epoxide, and melatonin, a new HPLC method was developed. Waters millennium 2010 chromatography manager with a 515 HPLC pump and Waters 24879 dual absorbance UV detector was used. A 25 microlitre of sample and standards were injected, and chromatographic separation was achieved by Merck C18 reverse phase column particle size 5 micro, 250 mm x 4 mm. It was quantitated at UV light 210 nm. The retention times of melatonin, CBZ-Epo, and CBZ were 6.3 min, 7.5 min, and 13.9 min respectively. The Mobile Phase used was water acetonitrile (7030), pH 3.0 adjusted with orthophosphoric acid at the flow rate of 1 ml/min. The limits of detection of melatonin, carbamazepine epoxide, and carbamazepine were 800, 500, and 1300 pg respectively.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Espectrofotometria Ultravioleta
/
Humanos
/
Carbamazepina
/
Criança
/
Cromatografia Líquida de Alta Pressão
/
Área Sob a Curva
/
Epilepsia
/
Melatonina
/
Anticonvulsivantes
/
Antioxidantes
Tipo de estudo:
Guia de Prática Clínica
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Physiol Pharmacol
Ano de publicação:
2006
Tipo de documento:
Artigo
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