Your browser doesn't support javascript.
loading
A comparison of conventional and radiometric methods for the assessment of anti-tubercular activity of drugs against Mycobacterium tuberculosis in mice and macrophage models.
Artigo em Inglês | IMSEAR | ID: sea-110561
ABSTRACT

BACKGROUND:

Presently, in vitro and in vivo screening of anti-tubercular drugs is a time-consuming exercise. Therefore, it is important to develop faster methods. MATERIAL AND

METHODS:

Towards this end, conventional plating and radiometric BACTEC methods of anti-tubercular screening were compared to determine the efficacy of anti-tubercular drugs (isoniazid and rifampicin) and morphine in Mycobacterium tuberculosis H37Rv-infected mice and macrophages.

RESULTS:

A linear correlation (R2 = 0.95) was observed between number of colony forming units (CFUs) and growth index (GI) values. BACTEC method was found to be faster and sensitive as compared to plating method. Further, BACTEC method, being a closed system, appeared to be less susceptible to microbial contamination and poses less biohazard.

CONCLUSION:

We conclude that BACTEC method can be employed for easy, precise, and rapid screening of anti-tubercular compounds and morphine in mice and macrophage models.
Assuntos
Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Radiometria / Rifampina / Baço / Feminino / Humanos / Masculino / Contagem de Colônia Microbiana / Testes de Sensibilidade Microbiana / Células Cultivadas / Técnicas Bacteriológicas Idioma: Inglês Ano de publicação: 2008 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Assunto principal: Radiometria / Rifampina / Baço / Feminino / Humanos / Masculino / Contagem de Colônia Microbiana / Testes de Sensibilidade Microbiana / Células Cultivadas / Técnicas Bacteriológicas Idioma: Inglês Ano de publicação: 2008 Tipo de documento: Artigo