Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity.
J Biosci
;
2003 Jun; 28(4): 379-82
Artigo
em Inglês
| IMSEAR
| ID: sea-110683
ABSTRACT
Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based on in vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fatores de Tempo
/
Besouros
/
Bioquímica
/
Encéfalo
/
Feminino
/
Difosfato de Adenosina
/
Trifosfato de Adenosina
/
Sensibilidade e Especificidade
/
Luciferina de Vaga-Lumes
/
Luciferases
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2003
Tipo de documento:
Artigo
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