Cytotoxicity of yellow sand in lung epithelial cells.
J Biosci
;
2003 Feb; 28(1): 77-81
Artigo
em Inglês
| IMSEAR
| ID: sea-110998
ABSTRACT
The present study was carried out to observe the cytotoxicity of yellow sand in comparison with silica and titanium dioxide in a rat alveolar type II cell line (RLE-6TN). Yellow sand (China Loess) was obtained from the loess layer in the Gunsu Province of China. The mean particle diameter of yellow sand was about 0.003 +/- 0.001 mm. Major elements of yellow sand were Si(27.7 +/- 0.6%), Al(6.01 +/- 0.17%), and Ca(5.83 +/- 0.23%) in that order. Silica and yellow sand significantly decreased cell viability and increased [Ca2+]i. All three particles increased the generation of H2O2. TiO2 did not change Fenton activity, while silica induced a slight increase of Fenton activity. In contrast, yellow sand induced a significant increase of Fenton activity. Silica, yellow sand and TiO2 induced significant nitrite formations in RLE-6TN cells. Silica showed the highest increase in nitrite formation, while yellow sand induced the least formation of nitrite. Silica and yellow sand increased the release of TNF-a. Based on these results, we suggest that yellow sand can induce cytotoxicity in RLE-6TN cells and reactive oxygen species, Fenton activity and reactive nitrogen species might be involved in this toxicity.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tamanho da Partícula
/
Ratos
/
Silício
/
Titânio
/
Linhagem Celular
/
Sobrevivência Celular
/
Cálcio
/
Fator de Necrose Tumoral alfa
/
Macrófagos Alveolares
/
Meios de Cultivo Condicionados
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2003
Tipo de documento:
Artigo
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