Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants.
J Biosci
;
2002 Mar; 27(2): 105-12
Artigo
em Inglês
| IMSEAR
| ID: sea-111031
ABSTRACT
The human gene TM7SF2 encodes a polypeptide (SR-1) with high sequence similarity to sterol C-14 reductase, a key sterol biosynthetic enzyme in fungi, plants and mammals. In Neurospora and yeast this enzyme is encoded by the erg-3 and erg24 genes respectively. In an effort to demonstrate sterol C-14 reductase activity for SR-1 we constructed six recombinant genes coding for chimeras of the Neurospora erg-3 and SR-1 protein sequences and tested them for complementation of the Neurospora erg-3 mutant. To our surprise, all the chimeras failed to complement erg-3. A few of the chimeric proteins were also tested against the yeast erg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings.
Texto completo:
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Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Oxirredutases
/
Proteínas Recombinantes de Fusão
/
Humanos
/
Dados de Sequência Molecular
/
Mutagênese Sítio-Dirigida
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
Oxirredutases atuantes sobre Doadores de Grupo CH-CH
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Teste de Complementação Genética
/
Animais
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2002
Tipo de documento:
Artigo
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