In vitro production of azadirachtin from cell suspension cultures of Azadirachta indica.
J Biosci
;
2008 Mar; 33(1): 113-20
Artigo
em Inglês
| IMSEAR
| ID: sea-111128
ABSTRACT
The present study aimed to elucidate the effect of nutritional alteration on biomass content and azadirachtin production in cell suspensions of the elite neem variety crida-8.Variations in total nitrogen availability in the medium in terms of different ratios of nitrate ammonium showed that the ratio 41 revealed a profound effect, leading to a 1.5-fold increase in the total extracellular azadirachtin production (5.59 mg/l) over the standard MS medium.Reduction in sucrose (15 mg/l) in the medium exhibited a reduction in biomass and absence of azadirachtin, whereas total phosphate reduction raised intracellular azadirachtin production (6.98 mg/l). An altered medium with a nitrate ammonium ratio of 41 coupled with complete elimination of phosphate enhanced biomass by 36% (59.36 g/l).
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Cinética
/
Células Cultivadas
/
Biomassa
/
Técnicas de Cultura de Células
/
Meios de Cultura
/
Azadirachta
/
Limoninas
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2008
Tipo de documento:
Artigo
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