The expression of GFP under the control of fibroin promotor in primary ovarian cells of Antheraea pernyi.
J Biosci
;
2003 Dec; 28(6): 691-5
Artigo
em Inglês
| IMSEAR
| ID: sea-111144
ABSTRACT
The fibroin promoter can stably express foreign gene in lepidopteran cells. Total RNA was extracted from the gland of silkworm, Antheraea pernyi and the transcription initiation site of fibroin gene of A. pernyi was identified by RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE). The expression vector (pGFP-N2/Fib) was constructed by use of replacing the CMV promoter with the fibroin promoter. The results of visual screening under a fluorescent inverted microscope and Western blot analysis indicated that the GFP gene was expressed in the primary cells of ovary origins from A. pernyi.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ovário
/
Bombyx
/
Feminino
/
DNA
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
Regiões Promotoras Genéticas
/
Proteínas de Fluorescência Verde
/
Eletroforese em Gel de Poliacrilamida
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2003
Tipo de documento:
Artigo
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