Evidence for lipopolysaccharide-induced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells.
J Biosci
;
2003 Feb; 28(1): 129-34
Artigo
em Inglês
| IMSEAR
| ID: sea-111192
ABSTRACT
Effect of lipopolysaccharide (LPS) on RAW264.7 macrophage cell line was studied. LPS-treated RAW264.7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 mg/ml), almost 70% RAW264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B 7.1, B 7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW 264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
Células Dendríticas
/
Ativação Linfocitária
/
Linfócitos T
/
Biomarcadores
/
Antígenos de Histocompatibilidade Classe II
/
Diferenciação Celular
/
Regulação para Cima
/
Linhagem Celular
/
Lipopolissacarídeos
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2003
Tipo de documento:
Artigo
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