Defence transcriptome profiling of Zingiber zerumbet (L.) Smith by mRNA differential display.
J Biosci
;
2008 Mar; 33(1): 81-90
Artigo
em Inglês
| IMSEAR
| ID: sea-111198
ABSTRACT
Soft rot is a serious disease in ginger (Zingiber of?cinale Roscoe),imposing a considerable economic loss annually in all ginger-producing countries.In this study,mRNA differential display was employed to identify genes whose expression was altered in a soft rot-resistant accession of Zingiber zerumbet (L.) Smith,a wild relative of ginger, in response to Pythium aphanidermatum (Edson) Fitzp.,which is the principal causative agent of soft-rot disease in ginger.Analysis using 68 primer combinations identified 70 differentially expressed transcript-derived fragments (TDFs),of which 34 TDFs were selected for further analysis following reverse northern screening.Cloning and sequence characterization of the 34 TDFs yielded a total of 54 distinct clones.Functional categorization of these clones revealed seven categories,of which the defence/stress/signalling group was the largest,with clones homologous to genes known to be actively involved in various pathogenesis-related functions in other plant species.The significance of these genes in relation to the resistance response in Z.zerumbet is discussed.This study has provided a pool of candidate genes for detailed molecular dissection of the defence mechanisms in Z.zerumbet and for accessing wild genetic resources for the transgenic improvement of ginger.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Doenças das Plantas
/
Pythium
/
Transcrição Gênica
/
RNA Mensageiro
/
Análise de Sequência de DNA
/
Genes de Plantas
/
Zingiber officinale
/
Perfilação da Expressão Gênica
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2008
Tipo de documento:
Artigo
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