Nano-composition of riboflavin-nafion functional film and its application in biosensing.
J Biosci
;
2008 Jun; 33(2): 279-87
Artigo
em Inglês
| IMSEAR
| ID: sea-111258
ABSTRACT
A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of -562 +/- 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 +/- 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 (micro)M.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Riboflavina
/
Polímeros de Fluorcarboneto
/
Microscopia Eletrônica de Varredura
/
Técnicas Biossensoriais
/
Microscopia Eletrônica de Transmissão
/
Nanoestruturas
/
Eletroquímica
/
Eletrodos
/
Ouro
/
Membranas Artificiais
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2008
Tipo de documento:
Artigo
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