Tyrosine phosphorylation of the human guanylyl cyclase C receptor.
J Biosci
;
2000 Dec; 25(4): 339-46
Artigo
em Inglês
| IMSEAR
| ID: sea-111272
ABSTRACT
Tyrosine phosphorylation events are key components of several cellular signal transduction pathways. This study describes a novel method for identification of substrates for tyrosine kinases. Co-expression of the tyrosine kinase EphB1 with the intracellular domain of guanylyl cyclase C (GCC) in Escherichia coli cells resulted in tyrosine phosphorylation of GCC, indicating that GCC is a potential substrate for tyrosine kinases. Indeed, GCC expressed in mammalian cells is tyrosine phosphorylated, suggesting that tyrosine phosphorylation may play a role in regulation of GCC signalling. This is the first demonstration of tyrosine phosphorylation of any member of the family of membrane-associated guanylyl cyclases.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fosforilação
/
Plasmídeos
/
Tirosina
/
Mapeamento de Peptídeos
/
Proteínas Recombinantes de Fusão
/
Humanos
/
Imunoglobulina G
/
Testes de Precipitina
/
Transdução de Sinais
/
Linhagem Celular
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2000
Tipo de documento:
Artigo
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