Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana.
J Biosci
;
2008 Mar; 33(1): 91-101
Artigo
em Inglês
| IMSEAR
| ID: sea-111306
ABSTRACT
A full-length cDNA encoding ribosome-inactivating/antiviral protein (RIP/AVP)from the leaves of Bougainvillea x buttiana was isolated.The cDNA consisted of 1364 nucleotides with an open reading frame (ORF)of 960 nucleotides encoding a 35.49 kDa protein of 319 amino acids.The deduced amino acid sequence has a putative active domain conserved in RIPs/AVPs and shows a varying phylogenetic relationship to the RIPs from other plant species.The deduced protein has been designated BBAP1 (Bougainvillea x buttiana antiviral protein1).The ORF was cloned into an expression vector and expressed in E.coli as a fusion protein of approximately 78 kDa.The cleaved and purified recombinant BBAP1 exhibited ribosome-inhibiting rRNA N-glycosidase activity,and imparted a high level of resistance against the tobacco mosaic virus (TMV).
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Antivirais
/
Filogenia
/
Proteínas de Plantas
/
Vírus do Mosaico do Tabaco
/
Proteínas Recombinantes de Fusão
/
Sinais Direcionadores de Proteínas
/
Dados de Sequência Molecular
/
Inibidores da Síntese de Proteínas
/
Sequência de Bases
/
Expressão Gênica
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2008
Tipo de documento:
Artigo
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