Comparison of the activities of wild type and mutant enhancing factor/mouse secretory phospholipase A2 proteins.
J Biosci
;
2002 Sep; 27(5): 489-94
Artigo
em Inglês
| IMSEAR
| ID: sea-111323
ABSTRACT
Enhancing factor (EF) protein, an isoform of secretory phospholipase A2 (PLA2), was purified as a modulator of epidermal growth factor from the small intestine of the Balb/c mouse. It was for the first time that a growth modulatory property of sPLA2 was demonstrated. Deletion mutation analysis of EF cDNA carried out in our laboratory showed that enhancing activity and phospholipase activity are two separate activities that reside in the same molecule. In order to study the specific amino acids involved in each of these activities, two site-directed mutants of EF were made and expressed in vitro. Comparison of enhancing activity as well as phospholipase A2 activity of these mutant proteins with that of wild type protein helped in identification of some of the residues important for both the activities
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Fosfolipases A
/
Humanos
/
Sequência de Bases
/
Linhagem Celular
/
Western Blotting
/
Northern Blotting
/
Mutagênese Sítio-Dirigida
/
Primers do DNA
/
Fosfolipases A2
/
Fosfolipases A2 do Grupo II
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS