Estimation of BCL-2 protein in carcinoma of the breast and its clinical correlation in locally advanced breast cancer.
J Cancer Res Ther
;
2007 Oct-Dec; 3(4): 207-10
Artigo
em Inglês
| IMSEAR
| ID: sea-111539
ABSTRACT
The change in expression of apoptotic markers (Bcl-2 and Bax proteins) brought about by various chemotherapeutic regimens is being used for its predictive value for assessing response to neoadjuvant chemotherapy (NACT) in locally advanced breast carcinoma (LABC). AIMS:
(1) Estimation of Bcl 2 expression in LABC, (2) Any change in Bcl 2 expression following chemotherapy in LABC, (3) Any relation of Bcl 2 estimation to changes in size of tumor, nodal status, age, and menopausal status. SETTINGS ANDDESIGN:
This was a prospective study of 120 cases of LABC. MATERIALS ANDMETHODS:
All cases were subjected to biopsy and the tissue was evaluated immunohistochemically for apoptotic marker Bcl-2 family protein. Three cycles of NACT were given at three-weekly intervals. Modified radical mastectomy was performed and the specimens were re-evaluated for any change in the Bcl-2 family protein. The clinical response and immunohistochemical response were correlated and compared. STATISTICALANALYSIS:
Coefficient of correlation was calculated by Pearson correlation coefficient (P-value).RESULTS:
Clinical response, as measured by reduction in the tumor size, was observed in 81 (67.5%) patients while immunohistochemical response was observed in 67 (55.8%) patients. Correlation between immunohistochemical and clinical response was found to be statistically significant (P = 0.02). Nodal response was seen in 72 (60%) patients. There were no patients in the N o group; 22 (53.7%) of the N 1 patients were down-staged to N o , while 19 (46.3%) remained N 1 . In patients with N 2 disease, 11 (13.9%) were down-staged to N o status, 39 (49.4%) were down-staged to N 1 status, and 29 (36.7%) did not show any response. Immunohistochemical response was observed in 67 (55.8%) patients. Correlation between immunohistochemical and nodal responses was also found to be statistically significant (P = 0.03).CONCLUSIONS:
This significant positive correlation between clinical and immunohistochemical responses highlights the importance of apoptotic marker Bcl-2 family protein in predicting the response of LABC to NACT.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Neoplasias da Mama
/
Idoso
/
Feminino
/
Humanos
/
Protocolos de Quimioterapia Combinada Antineoplásica
/
Doxorrubicina
/
Biomarcadores Tumorais
/
Estudos Prospectivos
/
Técnica Indireta de Fluorescência para Anticorpo
/
Proteínas Proto-Oncogênicas c-bcl-2
Tipo de estudo:
Estudo observacional
Idioma:
Inglês
Revista:
J Cancer Res Ther
Assunto da revista:
Neoplasms
/
Therapeutics
Ano de publicação:
2007
Tipo de documento:
Artigo
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