Analysis of mycobacterial strains by two-dimensional gel electrophoresis.
Artigo
em Inglês
| IMSEAR
| ID: sea-112020
ABSTRACT
This study pertains to analysis of the protein profile of different mycobacterial strains by two-dimensional gel electrophoresis (2DE). The strains were selected as they exhibit different phenotypic behaviour. TCA-acetone precipitated proteins were resolved by 2DE using immobilized pH gradient (IPG) strips. This study demonstrates that 2DE may be used as a tool for characterization of mycobacterial strains. Visual examination of the electrophoretograms was sufficient for characterization. Detailed characterization of specific proteins might lead to development of novel targets, diagnostic probes or sub-unit vaccine(s) against tuberculosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Proteínas de Bactérias
/
Humanos
/
Eletroforese em Gel Bidimensional
/
Mycobacterium smegmatis
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Ano de publicação:
2006
Tipo de documento:
Artigo
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