Use of sodium transfer tissue biosensor (STTB) for monitoring of marine toxic organism.
J Environ Biol
;
2004 Oct; 25(4): 431-6
Artigo
em Inglês
| IMSEAR
| ID: sea-113350
ABSTRACT
A highly sensitive sodium (Na+) transfer tissue biosensor (STTB) was designed using a frog bladder membrane to measure paralytic shellfish poisons (PSP). The STTB consists, of a Na+ electrode covered by the membrane, which was then integrated into a flow-through system for continuous measurements. In the absence of Na+ channel blocker, active transfer of Na+ occurred from inside to outside across the frog membrane. When the STTB was used to measure the Na+ -dependent dissociation of PSP, it was able to detect PSB at a level contained in a single cell. However, 5 fg or higher (100 cells or more) is needed for accurate and reproducible measurements. The toxicity obtained by the STTB was significantly correlated (r = 0.9449) to that determined by the HPLC. Therefore, the simple method of the STTB can be used not only to detect a low level PSP in toxic plankton populations, but also to monitor poisons in shellfish.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Anuros
/
Saxitoxina
/
Sódio
/
Tetrodotoxina
/
Transporte Biológico Ativo
/
Bexiga Urinária
/
Dinoflagellida
/
Técnicas Biossensoriais
/
Monitoramento Ambiental
/
Cromatografia Líquida de Alta Pressão
Idioma:
Inglês
Revista:
J Environ Biol
Ano de publicação:
2004
Tipo de documento:
Artigo
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