Heteropolymorphism of mitochondrial NADH dehydrogenase subunit 3 gene for the population analysis of chum salmon, Oncorhynchus keta.
J Environ Biol
;
2008 Jul; 29(4): 567-70
Artigo
em Inglês
| IMSEAR
| ID: sea-113825
ABSTRACT
Mitochondrial DNAs (mtDNAs) has been frequently used as genetic markers for the population genetic studies. In this study we used chum salmon (Oncorhynchus keta) from Korea, Japan andAmerica, and compared their mitochondrial NADH dehydrogenase subunit 3 (ND3) genes by DNA sequence analysis. Sequence variation was studied in the ND3 among total 11 individuals from three populations. The ND3 gene was amplified by PCR targeting parts of cytochrome oxidase III gene (COIII) and NADH dehydrogenase subunit 4L gene (ND4L). ND3 gene sequence, encoded 752 bps, presented some genetic variation in the chum salmon populations. The observed nucleotide variations inferred the distinct genetic differentiation of American salmons from Korean and Japanese chum salmons. Six sites of single nucleotide polymorphism (SNP) were explored in the ND3 locus. Denaturing gradient gel electrophoresis analysis also showed a clear heterogenous band in American salmons compared to Asian salmons.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Filogenia
/
Especificidade da Espécie
/
Variação Genética
/
DNA Mitocondrial
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Oncorhynchus keta
/
Polimorfismo de Nucleotídeo Único
/
Eletroforese
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
J Environ Biol
Ano de publicação:
2008
Tipo de documento:
Artigo
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