The inactive X chromosome in the human female is enriched in 5-methylcytosine to an unusual degree and appears to contain more of this modified nucleotide than the remainder of the genome.
J Genet
;
2003 Apr-Aug; 82(1-2): 13-6
Artigo
em Inglês
| IMSEAR
| ID: sea-114286
ABSTRACT
By employing a procedure that combines ELISA and photoacoustic spectroscopy, we have examined the content of 5-methylcytosine (m(5)C) in DNA of individuals who differed from one another in the number of X chromosomes in their genomes. The results show that the human inactive X chromosome (Xi) contains very high amounts of this modified nucleotide. We estimate that in the 46,XX female there is more m(5)C in Xi (~ 3.6 x 10(7)) than in all the remaining chromosomes put together (~ 2.1 x 10(7)). Our results also suggest that nearly one-fifth of all cytosines in Xi are methylated and that, in addition to CpG methylation, there is extensive non-CpG methylation as well.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Feminino
/
Humanos
/
DNA
/
Linfócitos
/
Genoma Humano
/
Repetições de Dinucleotídeos
/
Ilhas de CpG
/
Metilação de DNA
/
Cromossomos Humanos X
/
5-Metilcitosina
Idioma:
Inglês
Revista:
J Genet
Ano de publicação:
2003
Tipo de documento:
Artigo
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