Mutations in the S gene region of hepatitis B virus genotype D in Turkish patients.
J Genet
;
2007 Dec; 86(3): 195-201
Artigo
em Inglês
| IMSEAR
| ID: sea-114339
ABSTRACT
The S gene region of the hepatitis B virus (HBV) is responsible for the expression of surface antigens and includes the 'a'-determinant region. Thus, mutation(s) in this region would afford HBV variants a distinct survival advantage, permitting the mutant virus to escape from the immune system. The aim of this study was to search for mutations of the S gene region in different patient groups infected with genotype D variants of HBV, and to analyse the biological significance of these mutations. Moreover, we investigated S gene mutation inductance among family members. Forty HBV-DNA-positive patients were determined among 132 hepatitis B surface antigen (HbsAg) carriers by the first stage of seminested PCR. Genotypes and subtypes were established by sequencing of the amplified S gene regions. Variants were compared with original sequences of these serotypes, and mutations were identified. All variants were designated as genotype D and subtype ayw3. Ten kinds of point mutations were identified within the S region. The highest rates of mutation were found in chronic hepatitis patients and their family members. The amino acid mutations 125 (M -> T) and 127 (T -> P) were found on the first loop of 'a'-determinant. The other consequence was mutation inductance in a family member. We found some mutations in the S gene region known to be stable and observed that some of these mutations affected S gene expression.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Turquia
/
Feminino
/
Humanos
/
Masculino
/
DNA Viral
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Vírus da Hepatite B
/
Sequência de Aminoácidos
/
Homologia de Sequência de Aminoácidos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
J Genet
Ano de publicação:
2007
Tipo de documento:
Artigo
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