Cold-induced alteration in the global structure of the male sex chromosome of In1BM2(reinverted) of Drosophila melanogaster is associated with increased acetylation of histone 4 at lysine 16.
J Genet
;
2008 Dec; 87(3): 235-40
Artigo
em Inglês
| IMSEAR
| ID: sea-114483
ABSTRACT
In Drosophila melanogaster, dosage compensation occurs through hypertranscription of sex-linked genes in males. The hypertranscription involves acetylation of histone 4 at lysine 16 (H4K16) on amale X-chromosome, brought about by a histone acetyltransferase encoded by the dosage compensation gene, males absent on the first (mof). We report a phenomenon in the strain In(1)B(M2)(reinverted) of D. melanogaster where the global structure of the male X-chromosome can be altered at the third instar larval stage through a 4-h cold shock at 12+/-1 degrees C. We show that the cold shock results in a transient hyperacetylation of H4K16 and an increased expression of MOF. Control proteins H4 acetylated at lysine 5, and the dosage compensation gene msl-2, do not show any change in expression after cold shock. Cytology of the male X-chromosome at different time points during cold shock and recovery, suggests that the hyperacetylation of H4 at lysine 16 causes the X-chromosome to corkscrew into itself, thereby achieving the cold-induced change in the higher order structure of the male polytene X-chromosome. Our studies suggest a role for H4K16 in maintaining the structure of the male X-chromosome in Drosophila.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Acetilação
/
Cromossomo X
/
Feminino
/
Masculino
/
Histonas
/
Immunoblotting
/
Núcleo Celular
/
Temperatura Baixa
/
Drosophila melanogaster
/
Larva
Idioma:
Inglês
Revista:
J Genet
Ano de publicação:
2008
Tipo de documento:
Artigo
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