Markers for population genetic analysis of human plasmodia species, P. falciparum and P. vivax.
J Vector Borne Dis
;
2003 Sep-Dec; 40(3-4): 78-83
Artigo
em Inglês
| IMSEAR
| ID: sea-117901
ABSTRACT
Present report deals with the genetic diversity existing among the field isolates of Plasmodium falciparum and P. vivax in India. Isoenzymes and molecular markers were used to analyse field isolates of P. falciparum and P. vivax. High level of length polymorphism was observed in repeat nucleotide sequences of MSP-1, MSP-2 and GLURP in P. falciparum isolates and CSP, GAM-1 and MSP-3 alpha in P. vivax isolates. In study populations a high proportion of isolates (up to 60%) were comprised of more than one genetically distinct parasite type--multiclonal. Presence of identical allelic forms of enzyme and DNA variations in different geographical areas and in different years suggest that isolates belong to a single random mating population of P. vivax and P. falciparum. Observed random combination of alleles in the field isolates suggest the unlinked nature of loci studied. Study supports the feasibility of using molecular markers for the identification of recrudescence in P. falciparum from fresh infection.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Plasmodium falciparum
/
Plasmodium vivax
/
Polimorfismo Genético
/
Variação Genética
/
Humanos
/
Proteínas de Protozoários
/
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Malária Vivax
/
Malária Falciparum
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
J Vector Borne Dis
Assunto da revista:
Parasitology
/
Tropical Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo
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